TY  - JOUR
PY  - 2009//
TI  - A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry
JO  - Proteomics clinical applications
A1  - Sogawa, Kazuyuki
A1  - Satoh, Mamoru
A1  - Kodera, Yoshio
A1  - Tomonaga, Takeshi
A1  - Iyo, Masaomi
A1  - Nomura, Fumio
SP  - 821
EP  - 828
VL  - 3
IS  - 7
N2  - Objective markers are required to assess excessive alcohol consumption, which can lead to a various medical and social problems. In this study, we carried out serum peptidome analyses using the ClinProt(™) system, which consists of magnetic beads and MALDI-TOF/TOF MS, to find novel biomarkers of alcohol abuse in 16 chronic alcoholic patients that were hospitalized for a rehabilitation program. A total of 22 peaks were found to be significantly altered during abstinence. Out of these 22 peaks, 3 peaks that had an m/z of 3000 or less and substantial peak intensities were subjected to MS/MS analysis followed by a MASCOT search. The 1466 Da and the 1616 Da peptides were upregulated on admission and were identified as fragments of fibrinopeptide A and phosphorylated fibrinopeptide A, respectively. On the other hand, the 2660 Da peptide, which was downregulated on admission and increased during abstinence, was identified as a fragment of the fibrinogen α C chain. These peaks were not detectable by the SELDI-TOF MS ProteinChip(®) system analysis. The alterations in these peaks induced by alcohol abuse were also seen in γ glutamyltransferase nonresponders. These protein fragments may be additional biomarkers for excessive alcohol drinking.<p />  <p>Language: en</p>
LA  - en
SN  - 1862-8346
UR  - http://dx.doi.org/10.1002/prca.200800174
ID  - ref1
ER  -