TY  - JOUR
PY  - 2011//
TI  - The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects
JO  - Journal of applied toxicology
A1  - Carneiro, Raquel Felipe Vasconcelos
A1  - Nascimento, Nilberto Robson Falcão do
A1  - Costa, Paula Priscila Correia
A1  - Gomes, Victor Martins
A1  - Souza, Alex Jardelino Felizardo
A1  - Oliveira, Simone Cristina Buzzo
A1  - Santos Diz Filho, Eduardo Britto
A1  - Zara, Fernando José
A1  - Fonteles, Manassés Claudino
A1  - Oliveira Toyama, Daniela
A1  - Toyama, Marcos Hikari
A1  - Santos, Cláudia Ferreira
SP  - 720
EP  - 729
VL  - 31
IS  - 8
N2  - Phyllorhiza punctata (P. punctata) is a jellyfish native to the southwestern Pacific. Herewith we present the biochemical and pharmacological characterization of an extract of the tentacles of P. punctata. The tentacles were subjected to three freeze-thaw cycles, homogenized, ultrafiltered, precipitated, centrifuged and lyophilized to obtain a crude extract (PHY-N). Paralytic shellfish poisoning compounds such as saxitoxin, gonyautoxin-4, tetrodotoxin and brevetoxin-2, as well as several secretory phospholipase A(2) were identified. PHY-N was tested on autonomic and somatic neuromuscular preparations. In mouse vas deferens, PHY-N induced phasic contractions that reached a peak of 234 ± 34.7% of control twitch height, which were blocked with either 100 μm of phentolamine or 1 mm of lidocaine. In mouse corpora cavernosa, PHY-N evoked a relaxation response, which was blocked with either L-N(G) -Nitroarginine methyl ester (0.5 mm) or 1 mm of lidocaine. PHY-N (1, 3 and 10 μg ml(-1) ) induced an increase in tonus of the biventer-cervicis neuromuscular preparation that was blocked with pre-treatment of galamine (10 μm). Administration of 6 mg kg(-1) PHY-N intramuscularly produced death in broilers by spastic paralysis. In conclusion, PHY-N induces nerve depolarization and nonspecifically increases neurotransmitter release. Copyright © 2011 John Wiley & Sons, Ltd.<p />  <p>Language: en</p>
LA  - en
SN  - 0260-437X
UR  - http://dx.doi.org/10.1002/jat.1620
ID  - ref1
ER  -