TY  - JOUR
PY  - 2000//
TI  - A rapid method to detect dried saliva stains swabbed from human skin using fluorescence spectroscopy
JO  - Forensic science international
A1  - Soukos, N. S.
A1  - Crowley, K.
A1  - Bamberg, M. P.
A1  - Gillies, R.
A1  - Doukas, A. G.
A1  - Evans, R.
A1  - Kollias, N.
SP  - 133
EP  - 138
VL  - 114
IS  - 3
N2  - Saliva on skin is important in forensic trace evidence. If areas where saliva is present can be outlined, this may lead to DNA analysis and identification. This study describes a rapid and non-destructive method to detect dried saliva on the surface of the skin by fluorescence spectroscopy. Eighty-two volunteers deposited samples of their own saliva on the skin of their ventral forearm. A control sample of water was deposited at three different sites on the contralateral arm. Saliva and water control were then allowed to air-dry. Swab samples were taken from dried saliva and control sites and were dissolved in 0.1M KCl solution. Emission spectra were obtained from the solution and were characterized by a principal maximum at 345-355nm with excitation at 282nm. The fluorescence emission intensity was greater than background readings obtained from the control swab site in 80 of 82 volunteers (approximately 97.6%). The fluorescence profile of saliva samples were similar to those obtained from aqueous samples of pure amylase and tryptophan, an endogenous fluorophore in alpha-amylase. The presence of an emission peak at 345-355nm with excitation at 282nm could provide a strong presumptive indication of saliva deposition.<p /> <p>Language: en</p>
LA  - en
SN  - 0379-0738
UR  - http://dx.doi.org/
ID  - ref1
ER  -