TY - JOUR PY - 2011// TI - Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw JO - Sheng Wu Gong Cheng Xue Bao A1 - Ren, Shengtao A1 - Cheng, Keke A1 - Song, Andong A1 - Zhang, Jian'an SP - 592 EP - 597 VL - 27 IS - 4 N2 - In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 degrees C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L x h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 degrees C-50 degrees C.
Language: zh
LA - zh SN - 1000-3061 UR - http://dx.doi.org/ ID - ref1 ER -