TY  - JOUR
PY  - 1984//
TI  - An enzyme-linked immunoassay for detection of North American pit viper venoms
JO  - Journal of toxicology - clinical toxicology
A1  - Minton, S. A.
A1  - Weinstein, S. A.
A1  - Wilde, C. E.
SP  - 303
EP  - 316
VL  - 22
IS  - 4
N2  - We describe a method for immunodetection of North American pit viper venoms in clinical materials. Antibody-enzyme conjugates prepared against venoms of the western diamondback rattlesnake (Crotalus atrox), Mojave rattlesnake (Crotalus scutulatus), and copperhead (Agkistrodon contortrix) detect homologous venoms in concentrations of 0.1-.01 mcg/ml using a double antibody sandwich technique. Venoms of 10 additional species of U.S. pit vipers were detected in concentrations of 10 mcg/ml or less. Venoms of 4 species could not be detected at levels likely to be encountered in clinical situations. There are extensive cross-reactions between venoms of certain species, hence specific identification of a given venom cannot always be made. Venom usually can be detected at injection sites of experimental animals receiving intramuscular doses of 0.5-1.5 mg of venom but can rarely be detected in urine or plasma specimens. Venom was readily detected in specimens from experimental animals bitten by pit vipers of 6 species. The method is relatively rapid, simple, and inexpensive.<p /> <p>Language: en</p>
LA  - en
SN  - 0731-3810
UR  - http://dx.doi.org/
ID  - ref1
ER  -