TY  - JOUR
PY  - 2012//
TI  - Creatine reduces oxidative stress markers but does not protect against seizure susceptibility after severe traumatic brain injury
JO  - Brain research bulletin
A1  - Saraiva, André Luis Lopes
A1  - Ferreira, Ana Paula Oliveira
A1  - Silva, Luiz Fernando Almeida
A1  - Hoffmann, Maurício Scopel
A1  - Dutra, Fabrício Diniz
A1  - Furian, Ana Flávia
A1  - Oliveira, Mauro Schneider
A1  - Fighera, Michele Rechia
A1  - Royes, Luiz Fernando Freire
SP  - 180
EP  - 186
VL  - 87
IS  - 2-3
N2  - Achievements made over the last years have highlighted the important role of creatine in health and disease. However, its effects on hyperexcitable circuit and oxidative damage induced by traumatic brain injury (TBI) are not well understood. In the present study we revealed that severe TBI elicited by fluid percussion brain injury induced oxidative damage characterized by protein carbonylation, thiobarbituric acid reactive species (TBARS) increase and Na(+),K(+)-ATPase activity inhibition 4 and 8 days after neuronal injury. Statistical analysis showed that after TBI creatine supplementation (300mg/kg, p.o.) decreased the levels of protein carbonyl and TBARS but did not protect against TBI-induced Na(+),K(+)-ATPase activity inhibition. Electroencephalography (EEG) analysis revealed that the injection of a subconvulsant dose of PTZ (35mg/kg, i.p.), 4 but not 8 days after neuronal injury, decreased latency for the first clonic seizures and increased the time of spent generalized tonic-clonic seizures compared with the sham group. In addition, creatine supplementation had no effect on convulsive parameters induced by a subconvulsant dose of PTZ. Current experiments provide evidence that lipid and protein oxidation represents a separate pathway in the early post-traumatic seizures susceptibility. Furthermore, the lack of consistent anticonvulsant effect exerted by creatine in this early phase suggests that its apparent antioxidant effect does not protect against excitatory input generation induced by TBI.<p />  <p>Language: en</p>
LA  - en
SN  - 0361-9230
UR  - http://dx.doi.org/10.1016/j.brainresbull.2011.10.010
ID  - ref1
ER  -