TY  - JOUR
PY  - 2000//
TI  - Detection of exogenous intake of natural corticosteroids by gas chromatography/combustion/isotope ratio mass spectrometry: application to misuse in sport
JO  - Rapid communications in mass spectrometry
A1  - Bourgogne, E.
A1  - Herrou, V.
A1  - Mathurin, J. C.
A1  - Becchi, M.
A1  - de Ceaurriz, J.
SP  - 2343
EP  - 2347
VL  - 14
IS  - 24
N2  - A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5beta-androstanetrione before analysis by GC/C/IRMS. Significant differences in delta(13)C per thousand values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti-doping control, is proposed.<p /> <p>Language: en</p>
LA  - en
SN  - 0951-4198
UR  - http://dx.doi.org/10.1002/1097-0231(20001230)14:24<2343::AID-RCM171>3.0.CO;2-Z
ID  - ref1
ER  -