TY  - JOUR
PY  - 2012//
TI  - Silicon determination in human ventricular whole blood: a possible marker of drowning
JO  - Analytical biochemistry
A1  - Maraschi, Federica
A1  - Sturini, Michela
A1  - Speltini, Andrea
A1  - Orio, Francesco
A1  - Profumo, Antonella
A1  - Pierucci, Giovanni
SP  - 142
EP  - 146
VL  - 426
IS  - 2
N2  - Presented hereafter are the first results demonstrating that total silicon trace concentration in human ventricular whole blood may be used as a further marker in the diagnosis of drowning. The difference in silicon content between the left and the right ventricle resulted significantly higher for drowning cases than that from individuals who had not drowned. These findings were in full agreement with autoptic responses, supporting silicon as a marker of freshwater drowning. The procedure entails an alkaline microwave-assisted digestion using tetramethylammonium hydroxide (TMAH) in presence of H(2)O(2) followed by DRC-ICP-MS detection, whose accuracy was obtained for Seronorm whole blood reference material. Satisfying recoveries (91-98%) were gained on whole ventricular blood, with a silicon content lower than method detection limit (MDL), spiked at 5-7 μg g(-1) with materials consistent with drowning media constituents, i.e. fresh water plankton (CRM 414), silicon dioxide, diatomaceous earth powder and a silicon standard solution. Good within-lab reproducibility (4-10%) and sensitivity (MDL 0.46 μg g(-1)) were achieved too. The procedure has been applied to blood samples from 18 different real cases of death.<p />  <p>Language: en</p>
LA  - en
SN  - 0003-2697
UR  - http://dx.doi.org/10.1016/j.ab.2012.04.024
ID  - ref1
ER  -