TY  - JOUR
PY  - 2012//
TI  - General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus
JO  - Toxicon: Journal of the International Society on Toxinology
A1  - Costal-Oliveira, F.
A1  - Duarte, C. G.
A1  - Machado de Avila, R.
A1  - Melo, M. M.
A1  - Bordon, Karla de Castro Figueiredo
A1  - Arantes, E. C.
A1  - Paredes, N. C.
A1  - Tintaya, B.
A1  - Bonilla, C.
A1  - Bonilla, R. E.
A1  - Suarez, W. S.
A1  - Yarleque, A.
A1  - Fernandez, J. M.
A1  - Kalapothakis, E.
A1  - Chávez-Olórtegui, Carlos
SP  - 934
EP  - 942
VL  - 60
IS  - 5
N2  - This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD(50) determined was 0.1 mg/ Kg and 21.55 mg/Kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H.lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old Word-scorpion Androctonus australis Hector.<p />  <p>Language: en</p>
LA  - en
SN  - 0041-0101
UR  - http://dx.doi.org/10.1016/j.toxicon.2012.06.013
ID  - ref1
ER  -