TY  - JOUR
PY  - 2013//
TI  - Determination of paralytic shellfish poisoning toxins by HILIC-MS/MS coupled with dispersive solid phase extraction
JO  - Food chemistry
A1  - Zhuo, Liyang
A1  - Yin, Yuechun
A1  - Fu, Wusheng
A1  - Qiu, Bin
A1  - Lin, Zhenyu
A1  - Yang, Yiqiang
A1  - Zheng, Lemin
A1  - Li, Jianrong
A1  - Chen, Guonan
SP  - 115
EP  - 121
VL  - 137
IS  - 1-4
N2  - This paper describes the use of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) for the extraction, cleanup and detection of 10 paralytic shellfish toxins (PSP) in sea food by HILIC-MS/MS with positive ESI. Matrix matched calibration standards were used to compensate for matrix effects. The toxins were extracted with acetonitrile/water (90:10, v/v) containing 0.1% formic acid and cleaned by HLB and GCB sorbents. Qualitative and quantitative detection for the analytes were carried out under the multiple reaction monitoring (MRM) in positive ionization mode after chromatography separation on a TSK-gel Amide-80® column (150mm×2.0mm×3μm). Studies at three fortification levels for the toxins in the range of 8.1-225.5μg/kg gave mean recoveries from 71.3% to 104.6% with relative standard deviation (RSD)⩽15.8%. The limit of detection (LOD) was below the recommended regulatory limit of 170μgSTX(equ.)/kg and the proposed method fully meets the needs of daily monitoring.<p />  <p>Language: en</p>
LA  - en
SN  - 0308-8146
UR  - http://dx.doi.org/10.1016/j.foodchem.2012.10.010
ID  - ref1
ER  -