TY  - JOUR
PY  - 2010//
TI  - Kinetic electro membrane extraction under stagnant conditions--fast isolation of drugs from untreated human plasma
JO  - Journal of chromatography: A
A1  - Eibak, Lars Erik Eng
A1  - Gjelstad, Astrid
A1  - Rasmussen, Knut Einar
A1  - Pedersen-Bjergaard, Stig
SP  - 5050
EP  - 5056
VL  - 1217
IS  - 31
N2  - Amitriptyline, citalopram, fluoxetine, and fluvoxamine were isolated by electro membrane extraction (EME) from 70microl of untreated plasma (pH 7.4), through a supported liquid membrane (SLM) of 1-ethyl-2-nitrobenzene immobilized in the pores of a porous polypropylene hollow fiber, and into 30microl of 10mM HCOOH as acceptor solution inside the lumen of the hollow fiber. The driving force of the extraction was a 9V potential sustained over the SLM with a common battery, with the positive electrode placed in the plasma sample and the negative electrode placed in the acceptor solution. Extractions were performed under totally stagnant conditions with a very simple device for 1min (kinetic regime), and subsequently the acceptor solution was analyzed directly by liquid chromatography-mass spectrometry (LC-MS). Recoveries were 12, 13, 22, and 17% for fluoxetine, amitriptyline, citalopram, and fluvoxamine, respectively. Sample clean-up was comparable to reversed-phase solid-phase extraction (SPE), but EME required substantially less time than SPE. The time advantage of EME was further improved by parallel extraction of three samples (for 1min) with the same 9V battery. EME from plasma combined with LC-MS provided limits of quantification (S/N=10) in the range 0.4-2.3ng/ml, linearity in the range 1-1000ng/ml with r(2)-values of 0.998-0.999, and repeatability in the range 3.2-8.9% RSD in the mid-therapeutic window (100ng/ml).<p /> <p>Language: en</p>
LA  - en
SN  - 0021-9673
UR  - http://dx.doi.org/10.1016/j.chroma.2010.06.018
ID  - ref1
ER  -