TY  - JOUR
PY  - 2014//
TI  - Physiological and biochemical analysis to reveal the molecular basis for black widow spiderling toxicity
JO  - Journal of biochemical and molecular toxicology
A1  - Peng, Xiaozhen
A1  - Zhang, Yiya
A1  - Liu, Jinyan
A1  - Yu, Hai
A1  - Chen, Jia
A1  - Lei, Qian
A1  - Wang, Xianchun
A1  - Liang, Songping
SP  - 198
EP  - 205
VL  - 28
IS  - 5
N2  - The early research found that the spiderlings of black widow spider (Latrodectus tredecimguttatus) exhibited obvious toxicity to animals. The present work performed a systematical analysis of the aqueous extract of newborn black widow spiderlings. The extract was shown to contain 69.42% of proteins varying in molecular weights and isoelectric points. Abdominal injection of the extract into mice and cockroaches caused obvious poisoning symptoms as well as death, with LD50 being 5.30 mg/kg in mice and 16.74 µg/g in Periplaneta americana. Electrophysiological experiments indicated that the extract at a concentration of 10 µg/mL could completely block the neuromuscular transmission in isolated mouse nerve-hemidiaphragm preparations within 21 ± 1.5 min, and 100 µg/mL extract could inhibit a certain percentage of voltage-activated Na(+) , K(+) , and Ca(2+) channel currents in rat dorsal root ganglion neurons. These results demonstrate that the spiderlings are rich in neurotoxic components, which play important roles in the spiderling toxicity.<p />  <p>Language: en</p>
LA  - en
SN  - 1095-6670
UR  - http://dx.doi.org/10.1002/jbt.21553
ID  - ref1
ER  -