TY  - JOUR
PY  - 2014//
TI  - A highly rapid and simple competitive enzyme-linked immunosorbent assay for monitoring paralytic shellfish poisoning toxins in shellfish
JO  - Food chemistry
A1  - Kawatsu, Kentaro
A1  - Kanki, Masashi
A1  - Harada, Tetsuya
A1  - Kumeda, Yuko
SP  - 94
EP  - 98
VL  - 162
IS  - 
N2  - Using a streptavidin-coated well plate, a biotin-labelled anti-gonyautoxin 2/3 monoclonal antibody GT-13A, and a decarbamoyl saxitoxin-peroxidase conjugate, a direct competitive enzyme-linked immunosorbent assay (PSP-ELISA) was developed for monitoring paralytic shellfish poisoning (PSP) toxins in shellfish. This assay is simple to perform and can be completed in approximately 20min. The PSP-ELISA was compared to the mouse bioassay (MBA) for the detection of PSP toxins in shellfish samples (n=83) collected from the coast of Osaka Prefecture, Japan. When positive and negative results were indicated based on the regulatory limit for PSP toxins (4mouseunit(MU)/g of shellfish meat), the PSP-ELISA results showed a sensitivity of 100% (25 of 25) and a specificity of 89.7% (52 of 58 samples) compared to the MBA results. These results suggest that the PSP-ELISA could be used as a rapid and simple screening method prior to the MBA.<p /> <p>Language: en</p>
LA  - en
SN  - 0308-8146
UR  - http://dx.doi.org/10.1016/j.foodchem.2014.04.038
ID  - ref1
ER  -