TY  - JOUR
PY  - 1998//
TI  - A micro-analytical method for simultaneously quantifying benzoylecgonine, amphetamine, codeine and morphine in blood serum by means of GC/MS
JO  - Blutalkohol
A1  - Weinmann, Wolfgang
A1  - Renz, M
A1  - Pelz, C
A1  - Brauchle, P
A1  - Vogt, Sally
A1  - Pollak, Stefan
SP  - 195
EP  - 203
VL  - 35
IS  - 3
N2  - A GC/MS method for the simultaneous quantitation of amphetamine, benzoylecgonine, codeine and morphine using 0.1 mL serum has been developed. This method is based on a standard procedure for 1 mL serum samples (solid- phase extraction, derivatisation, GC/MS-analysis) which has now been modified for 0.1 mL serum-volumes. Linearity of calibration was achieved with standard GC/MS-conditions down to 1 to 5 ng/mL drug-concentration. For sample- preparation deuterated standards were added to spiked serum-samples, then solid-phase extraction and derivatisation of extracts with perfluoropropionic anhydride and pentafluoropropanol were performed. Analyses were carried out by means of GC/MS in Selected-Ion-Monitoring mode. The extraction efficiencies were between 86 and 98 % while the relative standard deviations of ten analyses were between 5.3 and 7.2 % (determined at a drug concentration of 10 ng/mL). With this method a GC/MS-analysis for verification of immunological results for amphetamine, benzoylecgonine, morphine and codeine can be achieved with as little as 0.1 mL serum-sample. However, this does not include tetrahydrocannabinol and its metabolites which cannot be detected by this method.<p />
LA  - de
SN  - 0006-5250
UR  - http://dx.doi.org/
ID  - ref1
ER  -