TY  - JOUR
PY  - 1986//
TI  - Intravital acellular hemolysis of extravascular erythrocytes. An experimental contribution to the determination of wound age
JO  - Zeitschrift fur Rechtsmedizin
A1  - Oehmichen, M.
A1  - Grüninger, H.
A1  - Norpoth, T.
SP  - 117
EP  - 131
VL  - 97
IS  - 2
N2  - The time-dependent acellular, destructive changes in erythrocytes was investigated in rabbits by subcutaneous, intraperitoneal, and subdural implantation of blood-filled diffusion chambers with 0.45 micron pores. The animals were killed at various intervals after implantation (7-24 h), and the erythrocytes in the capsules were examined by light and electron microscopy. The findings were quantified by counting 100 cells per preparation under the light microscope; changes in both matrix and membrane structure were recorded. Matrix changes and membrane variants were specified by electron microscopy. Time-dependent changes in erythrocyte matrix and membrane were established, which occurred at the various localizations in an identical sequence, but with different time intervals. The destructive changes were: an increase in loss of matrix, increase in membrane permeability, and decrease in membrane stability. The physiologic bases of and the conclusions for wound timing were discussed.<p /> <p>Language: de</p>
LA  - de
SN  - 0044-3433
UR  - http://dx.doi.org/
ID  - ref1
ER  -