TY  - JOUR
PY  - 2015//
TI  - Skeletal muscle protein breakdown remains elevated in pediatric burn survivors up to one-year post-injury
JO  - Shock
A1  - Chao, Tony
A1  - Herndon, David N.
A1  - Porter, Craig
A1  - Chondronikola, Maria
A1  - Chaidemenou, Anastasia
A1  - Abdelrahman, Doaa Reda
A1  - Bohanon, Fredrick J.
A1  - Andersen, Clark
A1  - Sidossis, Labros S.
SP  - 397
EP  - 401
VL  - 44
IS  - 5
N2  - Acute alterations in skeletal muscle protein metabolism are a well-established event associated with the stress response to burns. Nevertheless, the long-lasting effects of burn injury on skeletal muscle protein turnover are incompletely understood. This study was undertaken to investigate fractional synthesis (FSR) and breakdown (FBR) rates in skeletal muscle of pediatric burn patients (n = 42, >30% total body surface area burns) for up to 1 year after injury. Skeletal muscle protein kinetics were measured in the postprandial state following bolus injections of C6 and N phenylalanine stable isotopes. Plasma and muscle phenylalanine enrichments were quantified using gas chromatography-mass spectrometry. We found that the FSR in burn patients was 2- to 3-fold higher than values from healthy men previously reported in the literature (P ≤ 0.05). The FBR was 4- to 6-fold higher than healthy values (P < 0.01). Therefore, net protein balance was lower in burn patients compared with healthy men from 2 weeks to 12 months postinjury (P < 0.05). These findings show that skeletal muscle protein turnover stays elevated for up to 1 year after burn, an effect attributable to simultaneous increases in FBR and FSR. Muscle FBR exceeds FSR during this time, producing a persistent net negative protein balance, even in the postprandial state, which likely contributes to the prolonged cachexia seen in burned victims.<p />  <p>Language: en</p>
LA  - en
SN  - 1073-2322
UR  - http://dx.doi.org/10.1097/SHK.0000000000000454
ID  - ref1
ER  -