TY  - JOUR
PY  - 2015//
TI  - Traumatic brain injury accelerates kindling epileptogenesis in rats
JO  - Neurological research
A1  - Eslami, Mansoureh
A1  - Ghanbari, Elham
A1  - Sayyah, Mohammad
A1  - Etemadi, Fatemeh
A1  - Choopani, Samira
A1  - Soleimani, Mansoureh
A1  - Amiri, Zohreh
A1  - Hadjighassem, Mahmoudreza
SP  - 269
EP  - 274
VL  - 38
IS  - 3
N2  - OBJECTIVES: Traumatic brain injury (TBI) is a well-known cause of symptomatic epilepsy. In animal models of post-traumatic epilepsy (PTE), progression of trauma to epilepsy takes several weeks to months. Although this long process is similar to clinical PTE, it is costly and laborious. We used a combination of TBI and kindling as an accelerated animal model to develop epilepsy in much shorter period compared to that occurring in PTE. <br><br>METHODS: Traumatic brain injury was exerted to parieto-temporal cortex of anaesthetised rats by controlled cortical impact (CCI, 5 mm round tip, 4.5 mm/seconds velocity and 150 ms duration). Chemical kindling started 24 hours after CCI by intraperitoneal injection of 30 mg/kg pentylenetetrazole (PTZ) every other day until manifestation of three consecutive generalised seizures. Rapid electrical kindling of the amygdale began 1 week after TBI by exertion of 12 daily threshold stimuli (50 Hz mono-phasic square-wave stimulus of 1 ms per wave for 3 seconds) with 5 minutes interval between each stimulation until the rats became kindled. <br><br>RESULTS: Controlled cortical impact injury accelerated rate of both chemical and electrical kindling. Number of PTZ injections required for acquisition of generalised seizures decreased from 13.1 ± 1.6 in sham-operated animals to 7.1 ± 0.3 in traumatic rats (p < 0.05). The required number of stimuli to elicit electrically kindled focal and generalised seizures decreased from 24.0 ± 3.9 and 80 ± 6.5 in sham-operated animals to 6.6 ± 0.9 and 53 ± 6.5 in traumatic rats (p < 0.01), respectively. LIMITATIONS: Unlike the animal models of PTE in which recurrent seizures occur spontaneously after TBI, in our study, epilepsy is elicited by kindling stimulations. <br><br>DISCUSSION: Traumatic brain injury facilitates acquisition of epilepsy in both chemical and electrical kindling models. Combination of trauma and kindling can be considered as an inexpensive and time-saving animal model in PTE studies.<p />  <p>Language: en</p>
LA  - en
SN  - 0161-6412
UR  - http://dx.doi.org/10.1179/1743132815Y.0000000086
ID  - ref1
ER  -