TY  - JOUR
PY  - 2015//
TI  - Phenotype and functions of B cells in patients with acute brain injuries
JO  - Molecular immunology
A1  - Chenouard, Alexis
A1  - Chesneau, Mélanie
A1  - Braza, Faouzi
A1  - Dejoie, Thomas
A1  - Cinotti, Raphael
A1  - Roquilly, Antoine
A1  - Brouard, Sophie
A1  - Asehnoune, Karim
SP  - 350
EP  - 356
VL  - 68
IS  - 2 Pt A
N2  - BACKGROUND: Brain injuries (BI) induce a state of systemic immunosuppression, leading to a high risk of pneumonia. In this pilot study, we investigated the status of B cell compartment in BI patients. <br><br>METHODS: A prospective observational study was performed in 2 intensive care units in a university hospital. Blood samples were collected in 14 patients at day 1 and day 7 after acute BI. The phenotype and the ability of B cells to secrete IL-10 were compared to 11 healthy volunteers (HV). <br><br>RESULTS: Among the circulating lymphocytes, the frequency of B cells was significantly higher in BI patients compared to HV (p<0.001). B cells from BI patients displayed an activated profil on day 7 after BI, reflected by a significantly higher proportion of CD27(+) memory (p=0.01) and CD27(+) IgD(-) switched memory B cells (p=0.02), as well as a significantly higher blood level of IgA (p=0.001) and IgM (p<0.001) as compared to day 1. The frequency of IL-10 secreting B cells (IL-10(+) B cells) on day 1 and day 7 was significantly lower in BI patients compared to HV (p<0.05). Interestingly, we observed that all BI patients with high frequency of IL-10(+) B cells on day 1 displayed an episode of pneumonia, and had a longer duration of mechanical ventilation and ICU stay compared to BI patients with low proportion of IL-10(+) B cells. <br><br>CONCLUSION: This study provides an extensive description of the phenotype and function of B cells in BI patients. Our results suggest that IL-10(+) B cells could play a major role in immunosuppression after BI.<p />  <p>Language: en</p>
LA  - en
SN  - 0161-5890
UR  - http://dx.doi.org/10.1016/j.molimm.2015.09.001
ID  - ref1
ER  -