TY  - JOUR
PY  - 2015//
TI  - Brown spider (Loxosceles genus) venom toxins: evaluation of biological conservation by immune cross-reactivity
JO  - Toxicon: Journal of the International Society on Toxinology
A1  - Buch, Daniela Regina
A1  - Souza, Fernanda Nunes
A1  - Meissner, Gabriel Otto
A1  - Morgon, Adriano Marcelo
A1  - Gremski, Luiza Helena
A1  - Ferrer, Valéria Pereira
A1  - Trevisan-Silva, Dilza
A1  - Matsubara, Fernando Hitomi
A1  - Bóia-Ferreira, Mariana
A1  - Sade, Youssef Bacila
A1  - Chaves-Moreira, Daniele
A1  - Gremski, Waldemiro
A1  - Veiga, Silvio Sanches
A1  - Chaim, Olga Meiri
A1  - Senff-Ribeiro, Andrea
SP  - 154
EP  - 166
VL  - 108
IS  - 
N2  - Loxosceles spiders are responsible for serious human envenomations worldwide. The collection of symptoms found in victims after accidents is called loxoscelism and is characterized by two clinical conditions: cutaneous loxoscelism and systemic loxocelism. The only specific treatment is serum therapy, in which an antiserum produced with Loxosceles venom is administered to the victims after spider accidents. Our aim was to improve our knowledge, regarding the immunological relationship among toxins from the most epidemiologic important species in Brazil (L. intermedia, L. gaucho and L. laeta). Immunoassays using spider venoms and L. intermedia recombinant toxins were performed and their cross-reactivity assessed. The biological conservation of the main Loxosceles toxins (Phospholipases-D, Astacin-like metalloproteases, Hyaluronidase, ICK-insecticide peptide and TCTP-histamine releasing factor) were investigated. An in silico analysis of the putative epitopes was performed and is discussed on the basis of the experimental results. Our data is an immunological investigation in light of biological conservation throughout the Loxosceles genus. The results bring out new insights on brown spider venom toxins for study, diagnosis and treatment of loxoscelism and putative biotechnological applications concerning immune conserved features in the toxins.<p />  <p>Language: en</p>
LA  - en
SN  - 0041-0101
UR  - http://dx.doi.org/10.1016/j.toxicon.2015.09.041
ID  - ref1
ER  -