TY  - JOUR
PY  - 2016//
TI  - Comparative assay of Vipera ammodytes antivenom potency
JO  - Journal of enzyme inhibition and medicinal chemistry
A1  - Capitanescu, Cristian
A1  - Macovei Oprescu, Anca Monica
A1  - Supeanu, Alexandru
A1  - Coculescu, Bogdan Ioan
A1  - Strambu, Victor
A1  - Macovei, Radu Alexandru
A1  - Manole, Gheorghe
SP  - 1471
EP  - 1475
VL  - 31
IS  - 6
N2  - The finding of the most appropriate way to assess precisely the antivenom efficacy represents one of the major issues for antivenom standardization and success increasing of antivenom therapy. The efficacy of experimental Vipera ammodytes antivenom raised in sheep was determined using in vivo mouse lethality test, respectively, L-aminoacid oxidase, total proteinase and phospholipase A2 antienzymatic effectiveness. The values gained for the antivenom potency depend on the method of measure. So, some of the most toxic venom proteins own phospholipase A2 activity and provide the highest antivenom potency (lowest effective dose) values by antienzymatic assay method. This value is similar with total antiproteolytic antivenom potency value, but almost three times higher than value obtained by L-aminoacid oxidase (low toxic viper venom protein) antienzymatic assay method.<p />  <p>Language: en</p>
LA  - en
SN  - 1475-6366
UR  - http://dx.doi.org/10.3109/14756366.2016.1144598
ID  - ref1
ER  -