TY  - JOUR
PY  - 2016//
TI  - Region-specific alterations of A-to-I RNA editing of serotonin 2c receptor in the cortex of suicides with major depression
JO  - Translational psychiatry
A1  - Weissmann, D.
A1  - van der Laan, S.
A1  - Underwood, M. D.
A1  - Salvetat, N.
A1  - Cavarec, L.
A1  - Vincent, L.
A1  - Molina, F.
A1  - Mann, J. J.
A1  - Arango, V.
A1  - Pujol, J. F.
SP  - e878
EP  - e878
VL  - 6
IS  - 8
N2  - Brain region-specific abnormalities in serotonergic transmission appear to underlie suicidal behavior. Alterations of RNA editing on the serotonin receptor 2C (HTR2C) pre-mRNA in the brain of suicides produce transcripts that attenuate 5-HT2CR signaling by impairing intracellular G-protein coupling and subsequent intracellular signal transduction. In brain, the distribution of RNA-editing enzymes catalyzing deamination (A-to-I modification) shows regional variation, including within the cerebral cortex. We tested the hypothesis that altered pre-mRNA 5-HT2CR receptor editing in suicide is region-specific. To this end, we investigated the complete 5-HT2CR mRNA-editing profile in two architectonically distinct cortical areas involved in mood regulation and decision-making in a clinically well-characterized cohort of age- and sex-matched non-psychiatric drug-free controls and depressed suicides. By using an original biochemical detection method, that is, capillary electrophoresis single-stranded conformational polymorphism (CE-SSCP), we corroborated the 5-HT2CR mRNA-editing profile previously described in the dorsolateral prefrontal cortex (Brodmann area 9 (BA9)). Editing of 5-HT2CR mRNA displayed clear regional difference when comparing dorsolateral prefrontal cortex (BA9) and anterior cingulate cortex (BA24). Compared with non-psychiatric control individuals, alterations of editing levels of 5-HT2CR mRNA were detected in both cortical areas of depressed suicides. A marked increase in editing on 5-HT2CR was especially observed in the anterior cingulate cortex in suicides, implicating this cortical area in suicide risk. The results suggest that region-specific changes in RNA editing of 5-HT2CR mRNA and deficient receptor function likely contribute to the etiology of major depressive disorder or suicide.<p />  <p>Language: en</p>
LA  - en
SN  - 2158-3188
UR  - http://dx.doi.org/10.1038/tp.2016.121
ID  - ref1
ER  -