TY - JOUR
PY - 2016//
TI - Biochemical and kinetic evaluation of enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii
JO - Toxicon: Journal of the International Society on Toxinology
A1 - yue, Yang
A1 - Yu, Huahua
A1 - Li, Rongfeng
A1 - Xing, Ronge
A1 - Liu, Song
A1 - Li, Kecheng
A1 - Wang, Xueqin
A1 - Chen, Xiaolin
A1 - Li, Pengcheng
SP - 1
EP - 12
VL - 125
IS -
N2 - Jellyfish envenomations are emerging as an important public health concern occurred worldwide. In China, the situation is getting worse with numerous people stung by jellyfish Nemopilema nomurai (N. nomurai) and Cyanea nozakii (C. nozakii) in summer. However, the proteinaceous mixtures in nematocysts responsible for symptoms after jellyfish stings were scarcely characterized and understood in view of enzymatic constituents and toxicity. In the present study, enzymatic properties of jellyfish N. nomurai and C. nozakii nematocyst venom were analyzed biochemically and kinetically. The current data revealed that N. nomurai and C. nozakii nematocyst venom exhibited various enzymatic activities, of which metalloproteinases activity and PLA2s-like activity were predominant. Moreover, the catalytic activities of metalloproteinases and PLA2s-like were dependent on different physiochemical conditions such as temperature, pH and divalent ions. Kinetic profiling revealed their catalytic behaviors fitted the Michaelis-Menten equation under specific conditions.
FINDINGS suggested jellyfish nematocyst venom possessed diverse enzymatic constituents, which may underlie the extensively characterized bioactivities of jellyfish venom and human envenomations. Hence, our study will contribute to understanding the enzymatic constituents and toxicity of jellyfish nematocyst venom and may afford potential therapeutic targets for developing drugs for jellyfish stings.
Copyright © 2016. Published by Elsevier Ltd. Language: en
LA - en
SN - 0041-0101
UR - http://dx.doi.org/10.1016/j.toxicon.2016.11.005
ID - ref1
ER -