TY  - JOUR
PY  - 2016//
TI  - Increased 12/15-lipoxygenase leads to widespread brain injury following global cerebral ischemia
JO  - Translational stroke research
A1  - Yigitkanli, Kazim
A1  - Zheng, Yi
A1  - Pekcec, Anton
A1  - Lo, Eng H.
A1  - van Leyen, Klaus
SP  - 194
EP  - 202
VL  - 8
IS  - 2
N2  - Global ischemia following cardiac arrest is characterized by high mortality and significant neurological deficits in long-term survivors. Its mechanisms of neuronal cell death have only partially been elucidated. 12/15-lipoxygenase (12/15-LOX) is a major contributor to delayed neuronal cell death and vascular injury in experimental stroke, but a possible role in brain injury following global ischemia has to date not been investigated. Using a mouse bilateral occlusion model of transient global ischemia which produced surprisingly widespread injury to cortex, striatum, and hippocampus, we show here that 12/15-LOX is increased in a time-dependent manner in the vasculature and neurons of both cortex and hippocampus. Furthermore, 12/15-LOX co-localized with apoptosis-inducing factor (AIF), a mediator of non-caspase-related apoptosis in the cortex. In contrast, caspase-3 activation was more prevalent in the hippocampus. 12/15-lipoxygenase knockout mice were protected against global cerebral ischemia compared to wild-type mice, accompanied by reduced neurologic impairment. The lipoxygenase inhibitor LOXBlock-1 similarly reduced neuronal cell death both when pre-administered and when given at a therapeutically relevant time point 1 h after onset of ischemia. These findings suggest a pivotal role for 12/15-LOX in both caspase-dependent and caspase-independent apoptotic pathways following global cerebral ischemia and suggest a novel therapeutic approach to reduce brain injury following cardiac arrest.<p />  <p>Language: en</p>
LA  - en
SN  - 1868-4483
UR  - http://dx.doi.org/10.1007/s12975-016-0509-z
ID  - ref1
ER  -