TY  - JOUR
PY  - 2016//
TI  - Quantitative determination of cannabinoids in cannabis and cannabis products using ultra-high-performance supercritical fluid chromatography and diode array/mass spectrometric detection
JO  - Journal of forensic sciences
A1  - Wang, Mei
A1  - Wang, Yan-Hong
A1  - Avula, Bharathi
A1  - Radwan, Mohamed M.
A1  - Wanas, Amira S.
A1  - Mehmedic, Zlatko
A1  - van Antwerp, John
A1  - ElSohly, Mahmoud A.
A1  - Khan, Ikhlas A.
SP  - 602
EP  - 611
VL  - 62
IS  - 3
N2  - Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆(8) -THC, THCV, ∆(9) -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.<br><br>© 2016 American Academy of Forensic Sciences.<p />  <p>Language: en</p>
LA  - en
SN  - 0022-1198
UR  - http://dx.doi.org/10.1111/1556-4029.13341
ID  - ref1
ER  -