TY  - JOUR
PY  - 2018//
TI  - Cerebral alterations following experimental multiple trauma and hemorrhagic shock
JO  - Shock
A1  - Vogt, Nina
A1  - Herden, Christiane
A1  - Roeb, Elke
A1  - Roderfeld, Martin
A1  - Eschbach, Daphne
A1  - Steinfeldt, Thorsten
A1  - Wulf, Hinnerk
A1  - Ruchholtz, Steffen
A1  - Uhl, Eberhard
A1  - Schöller, Karsten
SP  - 164
EP  - 173
VL  - 49
IS  - 2
N2  - Multiple trauma (MT) associated with hemorrhagic shock (HS) might lead to cerebral hypoperfusion and brain damage. We investigated cerebral alterations using a new porcine MT/HS model without traumatic brain injury (TBI) and assessed the neuroprotective properties of mild therapeutic hypothermia. Male pigs underwent standardized MT with HS (45% or 50% loss of blood volume) and resuscitation after 90/120 minutes (T90/T120). In additional groups (TH90/TH120) mild hypothermia (33°C) was induced following resuscitation. Normothermic or hypothermic sham animals served as controls. Intracranial pressure (ICP), cerebral perfusion pressure (CPP), and cerebral oxygenation (PtiO2) were recorded up to 48.5 h. Serum protein S-100B and neuron specific enolase (NSE) were measured by ELISA. Cerebral inflammation was quantified on H&E-stained brain slices; Iba1, S100 and inducible nitric oxide synthase (iNOS) expression was assessed using immunohistochemistry. Directly after MT/HS, CPP and PtiO2 were significantly lower in T90/T120 groups compared to sham. After resuscitation both parameters showed a gradual recovery. Serum protein S-100B and NSE increased temporarily as a result of MT/HS in T90 and in T90/T120 groups, respectively. Cerebral inflammation was found in all groups. Iba1-staining showed significant microgliosis in T90 and T120 animals. iNOS-staining indicated a M1 polarization. Mild hypothermia reduced cerebral inflammation in the TH90 group, but resulted in increased iNOS activation. In this porcine long-term model we did not find evidence of gross cerebral damage when resuscitation was initiated within 120 minutes after MT/HS without TBI. However, trauma-related microglia activation and M1 microglia polarization might be a consequence of temporary hypoxia/ischemia and further research is warranted to detail underlying mechanisms. Interestingly mild hypothermia did not exhibit neuroprotective properties when initiated in a delayed fashion.<p />  <p>Language: en</p>
LA  - en
SN  - 1073-2322
UR  - http://dx.doi.org/10.1097/SHK.0000000000000943
ID  - ref1
ER  -