TY  - JOUR
PY  - 2017//
TI  - Forensic aspects of homicides by insulin overdose
JO  - Forensic science international
A1  - Tong, Fang
A1  - Wu, Rongqi
A1  - Huang, Wen
A1  - Yang, Yi
A1  - Zhang, Lin
A1  - Zhang, Biao
A1  - Chen, Xin
A1  - Tang, Xiaohui
A1  - Zhou, Yiwu
SP  - 9
EP  - 15
VL  - 278
IS  - 
N2  - Analysis of homicidal insulin overdose is a challenging task in forensic practice because of the difficulties in toxicological analysis as well as the elusive pathologic changes. We performed a detailed histopathologic examination on four autopsy cases involving insulin homicide, using H&E, immunohistochemistry (IHC) and immunofluorescence assays. Severe reactive astrocyte proliferation was obvious in the white matter of the cerebrum, corpus callosum, cerebellum and brain stem, especially in subcortical regions. We found a statistically significant increase in the number and total area of reactive astrocytes compared with controls (p<0.001). Insulin was detected at the injection sites of subcutaneous soft tissues by using IHC, luminescence immunoassay and immunofluorescence. Most insulin deposits were located in the gaps between adipocytes, and a few deposits were located in peripheral nerves and inflammatory cells. We also detected protamine in the skin tissues in two of the four cases. Our study revealed that the presence of insulin and/or protamine at the injection sites, along with severe reactive astrocyte proliferation, could help diagnose insulin overdose.<br><br>Copyright © 2017 Elsevier B.V. All rights reserved.<p />  <p>Language: en</p>
LA  - en
SN  - 0379-0738
UR  - http://dx.doi.org/10.1016/j.forsciint.2017.06.015
ID  - ref1
ER  -