TY  - JOUR
PY  - 2017//
TI  - PCR-based method for the detection of toxic mushrooms causing food-poisoning incidents
JO  - Shokuhin Eiseigaku Zasshi
A1  - Nomura, Chie
A1  - Masayama, Atsushi
A1  - Yamaguchi, Mizuka
A1  - Sakuma, Daisuke
A1  - Kajimura, Keiji
SP  - 132
EP  - 142
VL  - 58
IS  - 3
N2  - In this study, species-specific identification of five toxic mushrooms, Chlorophyllum molybdites, Gymnopilus junonius, Hypholoma fasciculare, Pleurocybella porrigens, and Tricholoma ustale, which have been involved in food-poisoning incidents in Japan, was investigated. Specific primer pairs targeting internal transcribed spacer (ITS) regions were designed for PCR detection. The specific amplicons were obtained from fresh, cooked, and simulated gastric fluid (SGF)-treated samples. No amplicons were detected from other mushrooms with similar morphology. Our method using one-step extraction of mushrooms allows rapid detection within 2.5 hr. It could be utilized for rapid identification or screening of toxic mushrooms.<p />  <p>Language: en</p>
LA  - en
SN  - 0015-6426
UR  - http://dx.doi.org/10.3358/shokueishi.58.132
ID  - ref1
ER  -