TY  - JOUR
PY  - 2018//
TI  - Establishment of a precise novel brain trauma model in a large animal based on injury of the cerebral motor cortex
JO  - Journal of neuroscience methods
A1  - Jiang, Jipeng
A1  - Dai, Chen
A1  - Niu, Xuegang
A1  - Sun, Hongtao
A1  - Cheng, Shixiang
A1  - Zhang, Zhiwen
A1  - Zhu, Xu
A1  - Wang, Yuting
A1  - Zhang, Tongshuo
A1  - Duan, Feng
A1  - Chen, Xuyi
A1  - Zhang, Sai
SP  - 95
EP  - 105
VL  - 307
IS  - 
N2  - BACKGROUND: Animal models are essential in simulating clinical diseases and facilitating relevant studies. NEW METHOD: We established a precise canine model of traumatic brain injury (TBI) based on cerebral motor cortex injury which was confirmed by neuroimaging, electrophysiology, and a series of motor function assessment methods. Twelve beagles were divided into control, sham, and model groups. The cerebral motor cortex was identified by diffusion tensor imaging (DTI), a simple marker method, and intraoperative electrophysiological measurement. Bony windows were designed by magnetic resonance imaging (MRI) scan and DTI. During the operation, canines in the control group were under general anesthesia. The canines were operated via bony window craniotomy and dura mater opening in the sham group. After opening of the bony window and dura mater, the motor cortex was impacted by a modified electronic cortical contusion impactor (eCCI) in the model group. <br><br>RESULTS: Postoperative measurements revealed damage to the cerebral motor cortex and functional defects. Comparisons between preoperative and postoperative results demonstrated that the established model was successful. COMPARISON WITH EXISTING METHOD(S): Compared with conventional models, this is the first brain trauma model in large animal that was constructed based on injury to the cerebral motor cortex under the guidance of DTI, a simple marker method, and electrophysiology. <br><br>CONCLUSION: The method used to establish this model can be standardized to enhance reproducibility and provide a stable and precise large animal model of TBI for clinical and basic research.<br><br>Copyright © 2018. Published by Elsevier B.V.<p />  <p>Language: en</p>
LA  - en
SN  - 0165-0270
UR  - http://dx.doi.org/10.1016/j.jneumeth.2018.06.025
ID  - ref1
ER  -