TY  - JOUR
PY  - 2018//
TI  - Mutagenic and DNA repair activity in traffic policemen: a case-crossover study
JO  - Journal of occupational medicine and toxicology
A1  - Ledda, Caterina
A1  - Loreto, Carla
A1  - Bracci, Massimo
A1  - Lombardo, Claudia
A1  - Romano, Gaetano
A1  - Cinà, Diana
A1  - Mucci, Nicola
A1  - Castorina, Sergio
A1  - Rapisarda, Venerando
SP  - e24
EP  - e24
VL  - 13
IS  - 
N2  - BACKGROUND: Emissions from vehicles are composed of heterogeneous mixtures of hazardous substances; several pollutants such as Polycyclic Aromatic Hydrocarbons (PAHs) are amongst the most dangerous substances detected in urban monitoring. A cohort of traffic policemen usually occupationally exposed to PAHs present in the urban environment were examined in order to assess the mutagenicity and DNA capacity repair. <br><br>METHODS: Seventy-two urban traffic policemen working in Catania's metropolitan area were enrolled in the study. Two spot urine samples were collected from each subject during the whole working cycle as follows: sample 1 (S1), pre-shift on day 1; sample 2 (S2) post-shift on day 6. 1-hydroxypyrene (1-OHP) was measured to serve as an indirect exposure indicator. Urinary mutagenic activity was assessed through the plate incorporation pre-incubation technique with S9, using YG1024 Salmonella typhimurium strain over-sensitive to PAH metabolite. Concentrations of urinary 8-oxodG were measured using liquid chromatography tandem mass spectrometry. <br><br>RESULTS: As regards the exposure to PAHs, results highlighted a statistically significant difference (<i>p</i> < 0.001) between pre-shift on day 1 and post-shift on day 6 levels. Mutagenic activity was detected in 38 (66%) workers on S1 and in 47 (81%) on S2. Also 8-oxodG analysis showed a statistically significant difference between S1 and S2 sampling. <br><br>CONCLUSIONS: This study demonstrated that occupational exposure to pollutants from traffic emission, assessed via 1-OHP measurements in urine, may lead to DNA repair and mutagenic activity, in line with other studies.<p />  <p>Language: en</p>
LA  - en
SN  - 1745-6673
UR  - http://dx.doi.org/10.1186/s12995-018-0206-9
ID  - ref1
ER  -