TY  - JOUR
PY  - 2018//
TI  - Biopanning of allergens from wasp sting patients
JO  - Bioscience reports
A1  - Chai, Lin
A1  - Yang, Xianyi
A1  - Liu, Mei
A1  - Liu, Chunyan
A1  - Han, Limei
A1  - Guo, Hui
A1  - Li, Changsheng
A1  - Sun, Yuwen
A1  - Li, Xiaoyan
A1  - Xiao, Min
A1  - Fang, Zhicheng
SP  - ePub
EP  - ePub
VL  - 38
IS  - 5
N2  - OBJECTIVE: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of this study was to systematically study the epitopes of wasp venom. <br><br>METHODS: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on 10 serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA. <br><br>RESULTS: A total of 35 specific potential wasp venom epitopes in 4 days were identified. Among them, 12 peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes. <br><br>CONCLUSION: The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.<br><br>©2018 The Author(s).<p />  <p>Language: en</p>
LA  - en
SN  - 0144-8463
UR  - http://dx.doi.org/10.1042/BSR20181113
ID  - ref1
ER  -