TY  - JOUR
PY  - 2019//
TI  - Dried blood spot compared to plasma measurements of blood-based biomarkers of brain injury in neonatal encephalopathy
JO  - Pediatric research
A1  - Massaro, An N.
A1  - Wu, Yvonne W.
A1  - Bammler, Theo K.
A1  - MacDonald, James W.
A1  - Mathur, Amit
A1  - Chang, Taeun
A1  - Mayock, Dennis
A1  - Mulkey, Sarah B.
A1  - van Meurs, Krisa
A1  - Afsharinejad, Zahra
A1  - Juul, Sandra
SP  - 655
EP  - 661
VL  - 85
IS  - 5
N2  - BACKGROUND: Data correlating dried blood spots (DBS) and plasma concentrations for neonatal biomarkers of brain injury are lacking. We hypothesized that candidate biomarker levels determined from DBS can serve as a reliable surrogate for plasma levels. DESIGN/METHODS: In the context of a phase II multi-center trial evaluating erythropoietin for neuroprotection in neonatal encephalopathy (NE), DBS were collected at enrollment ( < 24 h), day 2, 4, and 5. Plasma was collected with the first and last DBS. The relationship between paired DBS-plasma determinations of brain-specific proteins and cytokines was assessed by correlation and Bland-Altman analyses. For analytes with consistent DBS-plasma associations, DBS-derived biomarker levels were related to brain injury by MRI and 1-year outcomes. <br><br>RESULTS: We enrolled 50 newborns with NE. While S100B protein, tumor necrosis factor α, interleukin (IL)1 β, IL-6, IL-8 demonstrated significant DBS-plasma correlations, Bland-Altman plots demonstrated that the methods are not interchangeable, with a 2 to 4-fold error between measurements. No significant relationships were found between DBS levels of TNFα, IL-6, and IL-8 and outcomes. <br><br>CONCLUSION(S): Further work is needed to optimize elution and assay methods before using DBS specimens as a reliable surrogate for plasma levels of candidate brain injury biomarkers in NE.<p />  <p>Language: en</p>
LA  - en
SN  - 0031-3998
UR  - http://dx.doi.org/10.1038/s41390-019-0298-7
ID  - ref1
ER  -