TY  - JOUR
PY  - 2019//
TI  - A practical non-contact model to create standardized experimental burn wounds of any thickness: blue beam laser pointer for burn induction
JO  - Journal of burn care and research
A1  - Bingöl, Uğur Anıl
A1  - Oksüz, Sinan
A1  - Demiröz, Anıl
A1  - Arslan, Hakan
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - The objective of this study was to describe a predictable and easy-to-use model that can create standardized burn wounds. A 450 nm 1000 mW blue beam laser pointer was used to create burn wounds on the dorsal skin of 24 Sprague Dawley rats. 12 distinct areas of dorsal skin were pulsed for 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23 seconds with the help of a punched plastic card template. 3 groups of 8 animals were sacrificed immediately after, on the 3rd day and on the 7th day of the procedure and tissue samples were taken for histological evaluation and measurements. A second degree burn was obtained in all animals with 3 and 5 seconds of laser application on the same day, 3rd and 7th day measurements. 7 seconds of application resulted with a burn depth of 84.87% of dermis on the application day which deepened to involve the whole dermal layer on the 3rd and 7th day. 9 seconds and longer application times resulted 3rd degree burn wounds. Burn induction with blue beam laser pointer is an easy-to-use, predictable and safe model to create a standardized burn wound of desired thickness.<br><br>© American Burn Association 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.<p />  <p>Language: en</p>
LA  - en
SN  - 1559-047X
UR  - http://dx.doi.org/10.1093/jbcr/irz088
ID  - ref1
ER  -