TY  - JOUR
PY  - 2020//
TI  - Role of the serotonergic system in ethanol-induced aggression and anxiety: a pharmacological approach using the zebrafish model
JO  - European Neuropsychopharmacology
A1  - Müller, Talise E.
A1  - Ziani, Paola R.
A1  - Fontana, Barbara D.
A1  - Duarte, Tâmie
A1  - Stefanello, Flavia V.
A1  - Canzian, Julia
A1  - Santos, Adair R. S.
A1  - Rosemberg, Denis B.
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - Acute ethanol (EtOH) consumption exerts a biphasic effect on behavior and increases serotonin levels in the brain. However, the molecular mechanisms underlying alcohol-mediated behavioral responses still remain to be fully elucidated. Here, we investigate pharmacologically the involvement of the serotonergic pathway on acute EtOH-induced behavioral changes in zebrafish. We exposed zebrafish to 0.25, 0.5, 1.0% (v/v) EtOH for 1 h and analyzed the effects on aggression, anxiety-like behaviors, and locomotion. EtOH concentrations that changed behavioral responses were selected to the subsequent experiments. As a pharmacological approach, we used pCPA (inhibitor of tryptophan hydroxylase), WAY100135 (5-HT<sub>1A</sub> antagonist), buspirone (5-HT<sub>1A</sub> agonist), CGS12066A and CGS12066B (5-HT<sub>1B</sub> antagonist and agonist, respectively), ketanserin (5-HT<sub>2A</sub> antagonist) and (±)-DOI hydrochloride (5-HT<sub>2A</sub> agonist). All serotonergic receptors tested modulated aggression, with a key role of 5-HT<sub>2A</sub> in aggressive behavior following 0.25% EtOH exposure. Because CGS12066B mimicked 0.5% EtOH anxiolysis, which was antagonized by CGS12066A, we hypothesized that anxiolytic-like responses are possibly mediated by 5-HT<sub>1B</sub> receptors. Conversely, the depressant effects of EtOH are probably not related with direct changes on serotonergic pathway. Overall, our novel findings demonstrate a role of the serotonergic system in modulating the behavioral effects of EtOH in zebrafish. These data also reinforce the growing utility of zebrafish models in alcohol research and help elucidate the neurobiological mechanisms underlying alcohol abuse and associated complex behavioral phenotypes.<br><br>Copyright © 2020 Elsevier B.V. and ECNP. All rights reserved.<p />  <p>Language: en</p>
LA  - en
SN  - 0924-977X
UR  - http://dx.doi.org/10.1016/j.euroneuro.2019.12.120
ID  - ref1
ER  -