TY  - JOUR
PY  - 2020//
TI  - A new LC-MS/MS confirmation method for the determination of 17 drugs of abuse in oral fluid and its application to real samples
JO  - Forensic science international
A1  - Bassotti, Elisa
A1  - Merone, Giuseppe Maria
A1  - D'Urso, Annachiara
A1  - Savini, Fabio
A1  - Locatelli, Marcello
A1  - Tartaglia, Angela
A1  - Dossetto, Paolo
A1  - D'Ovidio, Cristian
A1  - de Grazia, Ugo
SP  - e110330
EP  - e110330
VL  - 312
IS  - 
N2  - A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation method for the direct analysis of 17 drugs starting from 200μL of diluted oral fluid (OF), in a single chromatographic run, was developed and validated. Cocaine, benzoylecgonine (BEG), cocaethylene, Δ-9-tetrahydrocannabinol (Δ-9-THC), buprenorphine, 6-acetylmorphine (6AM), morphine, codeine, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), methylenedioxyamphetamine (MDA), 3,4-Methylenedioxy-N-ethylamphetamine (MDE), ketamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB) were determined in a chromatographic run of 12min only with no sample pre-treatment, after the addition of 15 different internal standards (ISs). The method met all requirements in terms of linearity, accuracy (precision and trueness), recovery, and stability requested by FDA guidelines. Carry-over and interferences were negligible, as well as the matrix effects. LLOQs are below the limits defined by European guidelines and Italian national laws. The original oral fluid collections are stable at least six months at -20°C and one week at +4°C.<p />  <p>Language: en</p>
LA  - en
SN  - 0379-0738
UR  - http://dx.doi.org/10.1016/j.forsciint.2020.110330
ID  - ref1
ER  -