TY  - JOUR
PY  - 2020//
TI  - Validation of an analytical method for the simultaneous determination of 16 drugs and metabolites in hair in the context of driving license granting
JO  - Forensic science international
A1  - Müller, Victoria Vendramini
A1  - Hahn, Roberta Zilles
A1  - Lizot, Lilian de Lima Feltraco
A1  - Schneider, Anelise
A1  - da Silva, Cristiane Pereira
A1  - Gerbase, Fernando Engel
A1  - Pereira, Danilo
A1  - Linden, Rafael
A1  - Antunes, Marina Venzon
SP  - e110428
EP  - e110428
VL  - 315
IS  - 
N2  - The use of psychoactive substances has been associated with increased risk for traffic accidents. Hair testing has become a routine practice in clinical and forensic toxicological laboratories, with a unique perspective in the investigation of drug consumption. The study aimed to develop and validate a UHPLC-MS/MS method for the determination of multiple drugs in hair, to be used for toxicological examination in driving license granting. Sample preparation was a one-step liquid extraction of milled hair with methanol, which was incubated for 15h at 50°C. The chromatographic separation was performed in a reversed phase column, with a run time of 2.2min. Measured compounds were cocaine, benzoylecgonine, norcocaine, anhydroecgonine methyl ester, cocaethylene, amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, fenproporex, amfepramone, mazindol, codeine, morphine, 6-monoacetylmorphine, and tetrahydrocannabinol. The assay was linear for all substances (r>0.99), accurate (86.63-105.87 %), and precise, with a cv ranging from 1.9-13.5 % for intra-assay and 3.3-14.3 % for inter-assay. There was no significant carry over effect and the internal standard corrected matrix effect was minimal. The relative uncertainty percentages were below 9% for all the substances at cut-off values. The method was successfully applied to 50 hair samples from injured drivers, with 12% of positivity, including cocaine, MDMA and THC.<p />  <p>Language: en</p>
LA  - en
SN  - 0379-0738
UR  - http://dx.doi.org/10.1016/j.forsciint.2020.110428
ID  - ref1
ER  -