TY  - JOUR
PY  - 2020//
TI  - Pediatric burn survivors have long-term immune dysfunction with diminished vaccine response
JO  - Frontiers in immunology
A1  - Johnson, Blair Z.
A1  - McAlister, Sonia
A1  - McGuire, Helen M.
A1  - Palanivelu, Vetrichevvel
A1  - Stevenson, Andrew
A1  - Richmond, Peter
A1  - Palmer, Debra J.
A1  - Metcalfe, Jessica
A1  - Prescott, Susan L.
A1  - Wood, Fiona M.
A1  - Fazekas de St Groth, Barbara
A1  - Linden, Matthew D.
A1  - Fear, Mark W.
A1  - Fear, Vanessa S.
SP  - e1481
EP  - e1481
VL  - 11
IS  - 
N2  - Epidemiological studies have demonstrated that survivors of acute burn trauma are at long-term increased risk of developing a range of morbidities. The mechanisms underlying this increased risk remain unknown. This study aimed to determine whether burn injury leads to sustained immune dysfunction that may underpin long-term morbidity. Plasma and peripheral blood mononuclear cells were collected from 36 pediatric burn survivors >3 years after a non-severe burn injury (<10% total body surface area) and from age/sex-matched non-injured controls. Circulating cytokine and vaccine antibody levels were assessed using multiplex immunoassays and cell profiles compared using a panel of 40 metal-conjugated antibodies and mass cytometry. TNF-α (1.31-fold change from controls), IL-2 (1.18-fold), IL-7 (1.63-fold), and IFN-γ (1.18-fold) were all significantly elevated in the burn cohort. Additionally, burn survivors demonstrated diminished antibody responses to the diphtheria, tetanus, and pertussis vaccine antigens. Comparisons between groups using unsupervised clustering identified differences in proportions of clusters within T-cells, B-cells and myeloid cells. Manual gating confirmed increased memory T-regulatory and central memory CD4+ T-cells, with altered expression of T-cell, B-cell, and dendritic cell markers. <br><br>CONCLUSIONS: This study demonstrates a lasting change to the immune profile of pediatric burn survivors, and highlights the need for further research into post-burn immune suppression and regulation.<p />  <p>Language: en</p>
LA  - en
SN  - 1664-3224
UR  - http://dx.doi.org/10.3389/fimmu.2020.01481
ID  - ref1
ER  -