TY  - JOUR
PY  - 2022//
TI  - Two snakebite antivenoms have potential to reduce eswatini's dependency upon a single, increasingly unavailable product: results of preclinical efficacy testing
JO  - PLoS neglected tropical diseases
A1  - Menzies, Stefanie K.
A1  - Litschka-Koen, Thea
A1  - Edge, Rebecca J.
A1  - Alsolaiss, Jaffer
A1  - Crittenden, Edouard
A1  - Hall, Steven R.
A1  - Westhorpe, Adam
A1  - Thomas, Brent
A1  - Murray, James
A1  - Shongwe, Nondusimo
A1  - Padidar, Sara
A1  - Lalloo, David G.
A1  - Casewell, Nicholas R.
A1  - Pons, Jonathan
A1  - Harrison, Robert A.
SP  - e0010496
EP  - e0010496
VL  - 16
IS  - 9
N2  - BACKGROUND: Snakebite is a major public health concern in Eswatini, where treatment relies upon one antivenom-SAIMR Polyvalent. Although effective in treating snakebite, SAIMR Polyvalent is difficult to source outside its manufacturing country (South Africa) and is dauntingly expensive. We compared the preclinical venom-neutralising efficacy of two alternative antivenoms and SAIMR Polyvalent against the lethal and tissue-destructive effects of venoms from five species of medically important snakes using in vivo murine assays. The test antivenoms were 'Panafrican' manufactured by Instituto Clodomiro Picado and 'PANAF' manufactured by Premium Serums & Vaccines.     PRINCIPAL FINDINGS: In vivo murine preclinical studies identified both test antivenoms were equally or more effective than SAIMR Polyvalent at neutralising lethal and tissue-destructive effects of Naja mossambica venom. Both test antivenoms were less effective than SAIMR Polyvalent at neutralising the lethal effects of Bitis arietans, Dendroaspis polylepis, Hemachatus haemachatus and Naja annulifera venoms, but similarly effective at neutralising tissue damage induced by B. arietans and H. haemachatus venoms. In vitro immunological assays identified that IgG titres and toxin-specificities of the test antivenoms were comparable to SAIMR Polyvalent. Plasma clotting disturbances by H. haemachatus and N. mossambica were neutralised by the test antivenoms, whereas SAIMR Polyvalent failed to neutralise this bioactivity of N. mossambica venom. B. arietans SVMP activity was equally reduced by all three antivenoms, and H. haemachatus and N. mossambica PLA2 activities were neutralised by all three antivenoms. <br><br>CONCLUSIONS: While both Panafrican and PANAF antivenoms exhibited promising preclinical efficacies, both were less poly-specifically effective than SAIMR Polyvalent in these murine assays. The efficacy of these antivenoms against the lethal and tissue-destructive effects of N. mossambica venom, the most common biting species in Eswatini, identify that Panafrican and PANAF antivenoms offer effective alternatives to SAIMR Polyvalent for the treatment of snakebite in Eswatini, and potentially for neighbouring countries.<p />  <p>Language: en</p>
LA  - en
SN  - 1935-2727
UR  - http://dx.doi.org/10.1371/journal.pntd.0010496
ID  - ref1
ER  -