TY  - JOUR
PY  - 2022//
TI  - Muscle injury causes long-term changes in stem-cell DNA methylation
JO  - Proceedings of the National Academy of Sciences of the United States of America
A1  - Falick Michaeli, Tal
A1  - Sabag, Ofra
A1  - Fok, Rimma
A1  - Azria, Batia
A1  - Monin, Jonathan
A1  - Nevo, Yuval
A1  - Gielchinsky, Yuval
A1  - Berman, Benjamin P.
A1  - Cedar, Howard
A1  - Bergman, Yehudit
SP  - e2212306119
EP  - e2212306119
VL  - 119
IS  - 52
N2  - Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These de- and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis and are necessary for activation and regeneration. Local injury of one muscle elicits an almost identical epigenetic change in satellite cells from other muscles in the body, in a process mediated by circulating factors. Furthermore, this same methylation state is also generated in muscle stem cells (MuSCs) of female animals following pregnancy, even in the absence of any injury. Unlike the activation-induced expression changes, which are transient, the induced methylation profile is stably maintained in resident MuSCs and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for long-term adaptation.<p />  <p>Language: en</p>
LA  - en
SN  - 0027-8424
UR  - http://dx.doi.org/10.1073/pnas.2212306119
ID  - ref1
ER  -