TY - JOUR
PY - 2023//
TI - A fast and convenient sample preparation method for the analysis of cannabinoids in oral fluid
JO - Biomedical chromatography
A1 - Reinstadler, Vera
A1 - Huber, Susanne
A1 - Lierheimer, Stefan
A1 - Boettcher, Michael
A1 - Rüscher, Peter
A1 - Tanzer, Martin
A1 - Jenny, Kurt
A1 - Zoll, Adolf
A1 - Gruber, Wilhelm
A1 - Oberacher, Herbert
SP - ePub
EP - ePub
VL - ePub
IS - ePub
N2 - Cannabis is the world's most used illegal drug. The main psychoactive component is ∆-9-tetrahydrocannabinol (THC). To aid identification of cannabis-impaired individuals, a simple but effective workflow for reliable quantification of THC and its metabolites in oral fluid samples collected with the Greiner Bio-One Saliva Collection System is presented. Sampling involves rinsing the oral cavity with an extraction solution containing a citrate buffer stimulating salivary flow. Sample processing targeted the cannabinoid fraction interacting with proteins and other insoluble constituents that can be separated by centrifugation. Approximately 50% of the total cannabinoid amount included in oral fluid were recovered from the obtained pellet by extraction with acetonitrile. Liquid chromatography-tandem mass spectrometry was used for cannabinoid quantification. Fitness of the developed workflow for application in forensic and clinical cannabis testing was demonstrated by assessing multiple performance parameters, including selectivity, linearity, limits of quantification (LOQs), accuracy, precision, matrix effects, extraction recoveries, process efficiencies, and stability. Furthermore, sensitivity and specificity of the developed oral fluid-based cannabis test was demonstrated by analysing 195 samples collected either from opioid addicts or persons suspected of driving under the influence of drugs. The accuracy of identifying a person with presence of THC in blood was found to be 97.9%. Keywords: Cannabis impaired driving Language: en
LA - en
SN - 0269-3879
UR - http://dx.doi.org/10.1002/bmc.5651
ID - ref1
ER -