TY  - JOUR
PY  - 2023//
TI  - Simple and rapid detection of three amatoxins and three phallotoxins in human body fluids by UPLC-MS/MS and its application in 15 poisoning cases
JO  - Journal of analytical toxicology
A1  - Yang, Shuo
A1  - Wen, Di
A1  - Zheng, Fenshuang
A1  - Pu, Shanbai
A1  - Chen, Zhuonan
A1  - Chen, Mobing
A1  - Di, Bin
A1  - Liu, Wei
A1  - Shi, Yan
SP  - ePub
EP  - ePub
VL  - ePub
IS  - ePub
N2  - Amatoxins and phallotoxins are toxic cyclopeptides found in the genus Amanita and are among the predominant causes of foodborne sickness and poisoning-related fatalities in China. This study introduces and validates a simple, rapid, and cost-effective ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method for the simultaneous determination and quantification of α-amanitin, β-amanitin, γ-amanitin, phallisacin (PSC), phallacidin (PCD), and phalloidin (POD) in human blood and urine. Quick therapeutic decision-making is supported by a 9 min chromatographic separation performed on a Waters Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 µm) using a gradient of HPLC-grade water and methanol:0.005% formic acid. The analyte LOQs were 1-3 ng/mL in blood and 0.5-2 ng/mL in urine. Calibrations curves, prepared by spiking drug-free blood and urine, demonstrated acceptable linearity with mean correlation coefficients (r) greater than 0.99 for all phallotoxins and amatoxins. Acceptable intraday and interday precision (RSD <15%) and accuracy (bias, -4.8% to 13.0% for blood and -9.0% to 14.7% for urine) were achieved. The validated method was successfully applied to analyze 9 blood samples and 2 urine samples testing positive for amatoxins and/or phallotoxins. Amatoxins and/or phallotoxins were identified in each whole blood sample at a range of 1.12-5.63 ng/mL and in two urine samples from 1.01-9.27 ng/mL. The method has the benefits of simple sample preparation (protein precipitation) and wide analyte coverage, making it suitable for emergency quantitative surveillance toxicological analysis in clinics and forensic poisoning practice.<p />  <p>Language: en</p>
LA  - en
SN  - 0146-4760
UR  - http://dx.doi.org/10.1093/jat/bkad081
ID  - ref1
ER  -