TY  - JOUR
PY  - 2000//
TI  - Imidazoline receptor proteins are decreased in the hippocampus of individuals with major depression
JO  - Biological psychiatry
A1  - Piletz, J. E.
A1  - Zhu, H.
A1  - Ordway, G.
A1  - Stockmeier, C.
A1  - Dilly, G.
A1  - Reis, D.
A1  - Halaris, A.
SP  - 910
EP  - 919
VL  - 48
IS  - 9
N2  - BACKGROUND: A downregulation of I(2)-imidazoline binding sites has been reported in frontal cortices of depressed suicide victims, according to I(2)-radioligand binding and confirmed by Western blotting. We now report Western blots of imidazoline receptor proteins in hippocampi of subjects with and without depression at the time of death. <br><br>METHODS: Postmortem diagnoses were obtained from 17 cases of Axis I major depressive disorder and 17 cases without Axis I psychopathology. No psychotropic compounds were found in body fluids. Hippocampi were removed, sectioned, and assessed histologically. Throughout the analysis, each major depressive disorder sample was paired with a sample from a psychiatrically healthy subject based on equivalent life spans and postmortem delays. The antiserum was identical to that used in previous studies that reported a downregulation of cortical 29/30-kd imidazoline receptor-binding proteins in depression. <br><br>RESULTS: A triad of imidazoline receptor-binding protein bands (40-50 kd) was detected in the human hippocampus. Subjects with major depressive disorder had significantly less intensity in each imidazoline receptor-binding proteins band compared with control subjects (p =. 01 for overall bands). <br><br>CONCLUSIONS: The present results can be aligned with previous reports of downregulation of I(2)-radioligand binding sites in both cortices and platelets of depressed patients.<p /> <p>Language: en</p>
LA  - en
SN  - 0006-3223
UR  - http://dx.doi.org/10.1016/s0006-3223(00)00892-1
ID  - ref1
ER  -