TY  - JOUR
PY  - 2014//
TI  - A novel in-vitro model of human aortic valve mineralization
JO  - Journal of heart valve disease
A1  - Goncerz, G.
A1  - Tomaszewski, K.A.
A1  - Pasternak, A.
A1  - Głowacki, R.
A1  - Wróbel, A.
A1  - Rokita, E.
A1  - Podolec, P.
SP  - 545
EP  - 549
VL  - 23
IS  - 5
N2  - BACKGROUND AND AIM OF THE STUDY: The study aim was to develop an in-vitro model of human aortic valve homograft (AVH) mineralization, as a basis for further research on the subject of calcific aortic valve disease (CAVD).; METHODS: Human aortic valves were excised during autopsy (12-24 h post-mortem) from donors who had died due to accident or suicide. The mean (±SD) donor age was 32.5 ± 11.4 years. Under sterile conditions each aortic cusp (three cusps per valve) was cut in half to provide a total of 240 tissue samples. AVH mineralization was assessed in solutions with different Ca and P concentrations and CaxPO4 ionic product values. The impact of time on aortic valve mineralization rate was assessed using energy-dispersive X-ray fluorescence (ED-XRF).; RESULTS: The model showed that human AVH mineralization can be best observed in a solution with a CaxPO4 ionic product of 2.2 mmol2/l2. The optimum incubation time for observing AVH mineralization was 21 days.; CONCLUSION: A novel in-vitro AVH mineralization model was developed for use in future studies.<p /> <p>Language: en</p>
LA  - en
SN  - 0966-8519
UR  - http://dx.doi.org/
ID  - ref1
ER  -