TY  - JOUR
PY  - 2001//
TI  - Construction of salmonella stn-deficent mutants and detection of their virulence
JO  - Medical Journal of Chinese People's Liberation Army
A1  - Huang, Jianhua
A1  - Xu, Xinjing
SP  - 219
EP  - 221
VL  - 
IS  - 12
N2  - The plasmids of recombinant suicide vector containing the truncated stn gene and the deleted stn gene were constructed. The Salmonella stn-deficient mutant was prepared by homologous recombination between the mutant stn gene in the recombinant suicide vector and the wild-type gene in the chromosome of S. Typhimurium 2000. The detection of biological activity of stn gene production in those mutants indicated that Salmonella stn-deficient mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wild-type Salmonella. Upon oral challenge of mice, the fifty percent lethal dose of the Salmonella stn-deficient mutants was greater than that for the wild-type bacteria. Those studies showed that the stn gene is very important factor in the pathogenesis of salmonellosis.<p /> <p>Language: zh</p>
LA  - zh
SN  - 0577-7402
UR  - http://dx.doi.org/
ID  - ref1
ER  -