TY  - JOUR
PY  - 2015//
TI  - enterovirus-like RNA construct for colon cancer suicide gene therapy
JO  - Iran. Biomed. J.
A1  - Mahsa, Rasekhian
A1  - Ladan, Teimoori Toolabi
A1  - Safieh, Amini
A1  - Kayhan, Azadmanesh
SP  - 124
EP  - 132
VL  - 19
IS  - 3
N2  - In gene therapy, the use of RNA molecules as therapeutic agents has shown advantages over plasmid DNA, including higher levels of safety. However, transient nature of RNA has been a major obstacle in application of RNA in gene therapy. Here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3' non-translated region of Poliovirus to design an enterovirus-like RNA for the expression of a reporter gene [enhanced green fluorescent protein] and a suicide gene [thymidine kinase of herpes simplex virus]. The expression of these genes was evaluated by flow cytometry and cytotoxicity assay in human colorectal adenocarcinoma cell line [SW480]. We then armed RNA molecules with a target sequence for hsa-miR-143 to regulate their expression by microRNA [miRNA] mimics. The results showed effective expression of both genes by Entrovirus-like RNA constructs. The data also showed that the restoration of hsa-miR-143 expression in SW480 leads to a significant translation repression of the introduced reporter and suicide genes. Collectively, our data suggest the potential use of Entrovirus-like RNA molecules in suicide gene therapy. Additionally, as a consequence of the possible downregulated miRNA expression in cancerous tissues, a decreased expression of gene therapy constructs armed with target sequences for such miRNA in cancer tissue is expected<p /> <p>Language: en</p>
LA  - en
SN  - 1028-852X
UR  - http://dx.doi.org/
ID  - ref1
ER  -