TY  - JOUR
PY  - 2007//
TI  - Study on AdCD-UPRT suicide gene mediated on lung cancer A_(549) in vitro
JO  - Journal of Chongqing Medical University
A1  - Xu, Dan
SP  - 
EP  - 
VL  - 
IS  - 12
N2  - OBJECTIVE:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. <br><br>METHODS:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry. <br><br>RESULTS:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. <br><br>CONCLUSION:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.<p /> <p>Language: zh</p>
LA  - zh
SN  - 0253-3626
UR  - http://dx.doi.org/
ID  - ref1
ER  -