TY  - JOUR
PY  - 2002//
TI  - Study of encapsulation of suicide gene in poly(D,L-lactic-co-glycolic acid) nanoparticles and its characteristics in vitro
JO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
A1  - He, Qin
A1  - Zhang, Zhirong
A1  - Liu, Ji
A1  - Shu, Min
A1  - Zhang, Hua
A1  - Lu, Yi
A1  - Nie, Fei
A1  - Luo, Peng
SP  - 30
EP  - 33
VL  - 
IS  - 6
N2  - We constructed the AFP promotor, suicide gene and EGFP eukaryotic expression vector recombinant plasmid, and this plasmid DNA was encapsulated by biodegradable, biocompatible polymer PLGA to prepare nanoparticles. The results demonstrate that the mean diameter of DNA-PLGA-NP is 68 nm, the encapsulation ratio reaches to 80%, and the PLGA nanoparticles can protect plasmid DNA from digestion by deoxyribonuclease I (DNaseI) and sonication-induced shearing in vitro.<p /> <p>Language: zh</p>
LA  - zh
SN  - 1001-5515
UR  - http://dx.doi.org/
ID  - ref1
ER  -