TY  - JOUR
PY  - 1993//
TI  - tBOOH Acts as a Suicide Substrate for Catalase
JO  - Archives of biochemistry and biophysics
A1  - Pichorner, H.
A1  - Jessner, G.
A1  - Ebermann, R.
SP  - 258
EP  - 264
VL  - 300
IS  - 1
N2  - The effects of t-butyl hydroperoxide (tBOOH) on bovine liver catalase were investigated. tBOOH is accepted as a substrate of catalase and in the absence of hydrogen donors leads to a destruction of the enzyme via compound II formation. During the decomposition of this enzyme-substrate complex catalase serves as internal hydrogen donor which results in destruction of the enzyme. Evidence for this destruction is given by: - a decrease of the Soret band in the uv/vis spectrum, - iron release from the enzyme, - decrease of the catalatic activity of the enzyme measured by oxygen release from hydrogen peroxide. Hydrogen donors like NADH and o-dianisidine have been found to protect the enzyme from destruction by tBOOH but lead to a structural alteration of the enzyme, shown by alteration of the electrophoretic mobility. In the presence of the hydrogen donor tBOOH is completely reduced to t-butanol, which is thought to proceed in a peroxidase-like reaction. © 1993 Academic Press, Inc.<p /> <p>Language: en</p>
LA  - en
SN  - 0003-9861
UR  - http://dx.doi.org/10.1006/abbi.1993.1036
ID  - ref1
ER  -