TY  - JOUR
PY  - 1992//
TI  - Development and application of a radioimmunoassay to detect interleukin-1 in rat peripheral circulation
JO  - American journal of physiology
A1  - Derijk, R.
A1  - Berkenbosch, F.
SP  - E1092
EP  - 1098
VL  - 263
IS  - 6
N2  - We describe the development and application of a radioimmunoassay to detect circulating interleukin (IL)-1 beta concentrations in the rat. No IL-1 immunoreactivity above the detection limit of the assay (100 pg/ml) could be detected in plasma of control rats. In contrast, immunoreactive IL-1 was detected after intravenous administration of rat recombinant IL-1 beta (rrIL-1 beta) or bacterial lipopolysaccharide (LPS) to rats. The effect of LPS on plasma immunoreactive IL-1 concentrations was time and dose dependent. The immunoreactive IL-1 response to LPS was prevented by in vivo macrophage depletion induced by liposome-directed macrophage suicide technique. Gel filtration of plasma from LPS-treated rats revealed the presence of a high and a smaller molecular form of immunoreactive IL-1. The small molecular immunoreactive IL-1 peak coeluted with rrIL-1 beta and probably represents the 17-kDa form of IL-1 beta. In conclusion, our data support the hypothesis that IL-1 secreted by macrophages can act as a humoral signal molecule to induce the immunological, metabolic, and neuroendocrine changes in response to bacterial LPS.<p /> <p>Language: en</p>
LA  - en
SN  - 0002-9513
UR  - http://dx.doi.org/10.1152/ajpendo.2006.263.6.E1092
ID  - ref1
ER  -