TY  - JOUR
PY  - 1991//
TI  - Improved plant regeneration in maize callus cultures after pretreatment with dl-alpha-difluoromethylarginine
JO  - Plant Cell, Tissue and Organ Culture
A1  - Tiburcio, A.F.
A1  - Figueras, X.
A1  - Claparols, I.
A1  - Santos, M.
A1  - Torné, J.M.
SP  - 27
EP  - 32
VL  - 27
IS  - 1
N2  - The influence of a three-month-long pretreatment with dl-alpha-difluoromethylarginine (DFMA), an irreversible suicide inhibitor of arginine decarboxylase activity (ADC; EC 4.1.1.19), on plant regeneration, protein and polyamine (PA) composition of Zea mays callus cultures has been investigated. A four-fold increase in the number of regenerated plants is obtained after pretreatment with 0.5 mM DFMA. In addition, the regeneration frequency increases 3-fold in the treated calluses and the plants regenerated from such cultures are more developed than the untreated controls. The data obtained on protein and PA contents suggest that a senescence effect is exerted on the calluses grown in the presence of DFMA. However, after DFMA removal a rejuvenation effect occurs on the calluses that may explain the improvement of morphogenic capacity. This study indicates that DFMA pretreatment can be used to increase regeneration efficiency from maize callus cultures. © 1991 Kluwer Academic Publishers.<p /> <p>Language: en</p>
LA  - en
SN  - 1573-5044
UR  - http://dx.doi.org/10.1007/BF00048202
ID  - ref1
ER  -