TY  - JOUR
PY  - 2001//
TI  - A suicide-substrate mechanism for hydrolysis of beta-lactams by an anti-idiotypic catalytic antibody
JO  - FEBS letters
A1  - Lefèvre, S.
A1  - Debat, H.
A1  - Thomas, D.
A1  - Friboulet, A.
A1  - Avalle, B.
SP  - 25
EP  - 28
VL  - 489
IS  - 1
N2  - The catalytic mechanism of an anti-idiotypic antibody, 9G4H9, displaying a beta-lactamase activity was investigated. Kinetics experiments suggest that some penicillinic derivatives behave both as substrates and inactivators. Biochemical and immunological experiments strongly indicate that ampicillin may be regarded as a suicide substrate for hydrolysis by 9G4H9. The anti-idiotypic network appears as a way to create enzyme mimics with modified catalytic activities.<p /> <p>Language: en</p>
LA  - en
SN  - 0014-5793
UR  - http://dx.doi.org/10.1016/s0014-5793(01)02075-0
ID  - ref1
ER  -